Categories
2011

Designing peptide‐based FSL constructs to create Miltenberger kodecytes

Summary: Review of the principles for making peptide based-FSL constructs

Henry SM, Komarraju S, Heathcote D, Rodinov I. Designing peptide-based FSL constructs to create Miltenberger kodecytes. ISBT Science Series (2011); 6(2): 306-312

DOI: 10.1111/j.1751-2824.2011.01505.x

Categories
2011

A novel VSV/HIV pseudotype approach for the study of HIV microbicides without requirement for level 3 biocontainment

Summary: Toronto University show prevention of HIV infection of cells with FSL-GB3

Harrison AL, Henry S, Mahfoud R, Manis A, Albertini A, Gaudin Y, Lingwood CA, and Branch DR. A novel VSV/HIV pseudotype approach for the study of HIV microbicides without requirement for level 3 biocontainment. Future Virology (2011); 6(10): 1241-1259

DOI: 10.2217/fvl.11.88

Categories
2011

Fluorescein and radiolabeled Function-Spacer-Lipid constructs allow for simple in vitro and in vivo bioimaging of enveloped virions

Summary: Mayo Clinic shows FSL-fluorescent and FSL-radiolabeling of virus and bioimaging of labeled virus in mice

Hadac EM, Federspiel MJ, Chernyy E, Tuzikov A, Korchagina E, Bovin NV, Russell S, Henry SM. Fluorescein and radiolabeled Function-Spacer-Lipid constructs allow for simple in vitro and in vivo bioimaging of enveloped virions. J Virological Methods (2011); 176(1-2): 78-84

DOI:10.1016/j.jviromet.2011.06.005

Categories
2011

FSL Constructs: A Simple Method for Modifying Cell/Virion Surfaces with a Range of Biological Markers Without Affecting their Viability

Summary: Video and protocol showing generic use of Kode™ Technology – sponsored by Sigma-Aldrich

Blake DA, Bovin NV, Bess D, Henry SM. FSL Constructs: A Simple Method for Modifying Cell/Virion Surfaces with a Range of Biological Markers Without Affecting their Viability. J Visualized Experiments (2011) Aug 5; (54): e3289

DOI: 10.3791/3289

Categories
2011

Flow cytometry evaluation of red blood cells mimicking naturally occurring ABO subgroups after modification with variable amounts of function‐spacer‐lipid A and B constructs

Summary: Flow cytometry of kodecytes made to mimic antigen strengths of ABO cells with weak antigen expression

Hult AK, Frame T, Chesla S, Henry S, Olsson ML. Flow cytometry evaluation of red blood cells mimicking naturally-occurring ABO subgroups following modification with variable amounts of function-spacer-lipid A and B constructs. Transfusion (2011); 52(2): 247-251

DOI: 10.1111/j.1537-2995.2011.03268.x

Categories
2011

An improved Fc function assay utilizing CMV antigen‐coated red blood cells generated with synthetic function–spacer–lipid constructs

Summary: FSL-CMV peptides were used to create an assay for determining Fc functionality of IgG prepared for in intravenous infusion

Georgakopoulos T, Komarraju S, Henry S, Bertolini J. An improved Fc function assay utilising CMV antigen-coated red blood cells generated with synthetic Function-Spacer-Lipid constructs. Vox Sanguinis (2012); 102(1): 72-78

DOI: 10.1111/j.1423-0410.2011.01512.x

Categories
2011

In vivo neutralization of anti‐A and successful transfusion of A antigen–incompatible red blood cells in an animal model

Summary: Circulating antibodies were neutralised with FSLs allowing for incompatible transfusion in mice

Oliver C, Blake D, Henry S. In vivo neutralization of anti-A and successful transfusion of A antigen-incompatible red cells in an animal model. Transfusion (2011); 51(12): 2664-2675

DOI: 10.1111/j.1537-2995.2011.03184.x

Categories
2011

Prevalence and specificity of red‐blood‐cell antibodies in a multiethnic South and East Asian patient population and influence of using novel MUT+Mur+ kodecytes on its detection

Summary: Independent validation of Kode™ Miltenberger product

V. S. Nadarajan, A. A. Laing, S. M. Saad & M. Usin. Prevalence and specificity of red-blood-cell antibodies in a multiethnic South and East Asian patient population and influence of using novel MUT+Mur+ kodecytes on its detection. Vox Sang. (2011) Jan;102(1):65-71.

DOI: 10.1111/j.1423-0410.2011.01507.x

Categories
2011

Modeling transfusion reactions and predicting in vivo cell survival with kodecytes

Summary: FSL biotin labeled red cells can be used to determine in vivo cell survival and allow for retrieval of cells from the circulation

Oliver C, Blake D, Henry S. Modeling transfusion reactions and predicting in vivo cell survival with kodecytes. Transfusion (2011); 51(8): 1723-1730

DOI: 10.1111/j.1537-2995.2010.03034.x