Summary: Here we describe an alternative approach of influenza virus labeling that utilizes Function-Spacer-Lipid (FSL) constructs that can be gently inserted into the virus membrane.
Ilyushina, Natalia A., et al. “Labeling of influenza viruses with synthetic fluorescent and biotin-labeled lipids.” VIROLOGICA SINICA (2014); 29(4): 199-210.
Summary: In the absence of natural FORS1-positive red blood cells (RBCs), kodecytes were created with synthetic disaccharide and pentasaccharide Forssman function-spacer-lipid (FSL) constructs, Fsdi-kodecytes, and FORS1-kodecytes, respectively. FSL constructs were also applied to solid surfaces and used in solid-phase enzyme immunoassays.
Barr, Katie, Elena Korchagina, Inna Popova, Nicolai Bovin, and Stephen Henry. “Monoclonal anti‐A activity against the FORS1 (Forssman) antigen.” Transfusion (2015); 55(1): 129-136.
Mapping of MoAbs with kodecytes and printed FSL constructs revealed a series of broad recognition patterns. All ABO MoAbs tested were reactive with the RBC dominant Type 2 ABO antigens. Unexpectedly some anti-A reagents were reactive against the B Type 1 antigen, while others were poorly reactive with trisaccharide antigens.
Barr, Katie, Elena Korchagina, Ivan Ryzhov, Nicolai Bovin, and Stephen Henry. “Mapping the fine specificity of ABO monoclonal reagents with A and B type‐specific function‐spacer‐lipid constructs in kodecytes and inkjet printed on paper.” Transfusion (2014); 54(10): 2477-2484.